|I. General information|
|Background:||The hTERT-HPNE cell line was developed from human pancreatic duct by transduction with a retroviral expression vector (pBABEpuro) containing the hTERT gene. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings Ref.|
|Species:||Homo sapiens, human|
|Gender:||Male， 52 years|
|DNA Profile:||D5S818: 11
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|Culture Medium:||DMEM+25%M3 medium+5%FBS+10ng/ml EGF
We strongly suggest to purchase the complete medium from Cobioer.
|Comments:||Exposing hTERT-HPNE cells to sodium butyrate and 5-aza-2'-deoxycytidine lead to the formation of pancreatic ductal cells marked by the expression of p-glycoprotein (multidrug resistance (MDR-1)), carbonic anhydrase II, and the cytokeratins 7, 8, and 19.
hTERT-HPNE cells were found to have properties of the intermediary cells formed during acinar-to-ductal metaplasia, which included their undifferentiated phenotype, expression of Nestin and, evidence of active Notch signaling Ref.
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