|I. General information|
|Background:||The cell line was established by ATCC in 2003 from normal human foreskin pooled from two individuals.|
|Species:||Homo sapiens, human|
We strongly suggest to purchase the complete medium from Cobioer.
|Comments:||The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated (SCRC-1041.1) and treated the cells with Mitomycin C (SCRC-1041.2) for use as a feeder layer. If the HFFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 50 (P50). It is recommended that the feeder cells be plated 24 hours before use at 5X104 cells/cm2 in order to obtain a supportive monolayer for stem cell growth.
For more information, please contact Cobioer (4008-750-250).