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15P-1(小鼠睾丸上皮细胞)

科研细胞

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15P-1(小鼠睾丸上皮细胞)

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产品编号
CBP60908
货号:
CBP60908
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
睾丸
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CBP60908
I. General information 
Synonyms: 15P-1
Background: The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium.Ref
Species: Mus musculus, transgenic, mouse, transgenic
Tissue: testis
Gender: male, 6 months
Growth Mode: adherent
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium. Ref
This line exhibits characteristics of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. Ref
15P-1 cultures express phagocytic activity evidenced by the internalization of latex beads and the disposal of dead cells. Interaction with germ cells enhances the phagocytic activity of 15P-1 cells. Ref
The cells support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. Ref
When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generate tetrads of haploid cells with the morphology of round spermatids and initiate protamine transcription. Ref
The 15P-1 cell line releases protease-sensitive material with broad-spectrum antibacterial activity. The activity of 15P-1 culture medium is increased 10-fold to 50-fold in the presence of fractions enriched in round spermatids and of nerve growth factor. Ref
The KL protein, a ligand of the Kit receptor, is expressed in Sertoli cells and in 15P-1 cells. Ref
For more information, please contact Cobioer (4008-750-250).
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