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32D(小鼠IL-3依赖性细胞)

科研细胞

科研细胞

药靶细胞

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32D(小鼠IL-3依赖性细胞)

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产品编号
CBP60995
货号:
CBP60995
品牌:
Cobioer
规格:
T-25 Flask
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CBP60995
I. General information 
Synonyms: 32D
Background: established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent
Species: murine (mouse) (Mus musculus)
Tissue: bone 
Disease: bone marrow
Gender: N/A 
Morphology: mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent;
Growth Mode: N/A 
Doubling Time: ca. 15 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: IMDM + 10% FBS + 10% vol conditioned medium of cell line WEHI-3 OR +100ng/ml IL-3(R&D)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b +, F4/80 (+); 
Fingerprint: N/A 
Species: originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding
Cytogenetics: murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers
Virus   ELISA: reverse transcriptase negative; PCR: SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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