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SVEC4-10(小鼠淋巴内皮细胞(SV40转化))

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SVEC4-10(小鼠淋巴内皮细胞(SV40转化))

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产品编号
CBP60997
货号:
CBP60997
品牌:
Cobioer
规格:
T-25 Flask
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CBP60997
I. General information 
Synonyms: SVEC4-10
Background: SVEC4-10 is an endothelial cell line derived by SV40 (strain 4A) transformation of endothelial cells from axillary lymph node vessels.
Species: Mus musculus, mouse
Tissue: axillary lymph node/vascular epithelium
Gender: male, adult
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 24 to 30 hrs
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: H-2 K; Factor VIII related antigen; VCAM
Receptor Expression: high affinity receptors for low density lipoprotein (LDL)
Tumor Formation: Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks
Comments:  They grow indefinitely without special additives and are well differentiated, responding like normal endothelial cells to some interleukins and to extracellular matrix signals for tube-like differentiation.
When grown on a synthetic basement-like membrane, SVEC4-10 forms branching tube-like networks.
They specifically bind mouse lymphocytes in vitro.
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, and are susceptible to lysis by anti SV40 H-2 k CTL clones.
Interferon gamma (IFN-gamma) induces expression of MHC class II antigen in a time course identical to that of normal endothelial cells.
The cells express vascular cell adhesion molecule (VCAM), and stain positively for SV40 T antigen.
Tumor necrosis factor alpha (TNF alpha) induces SVEC4-10 to undergo reversible transition to a spindle shaped morphology.
SVEC4-10 is the parental line for a series of endothelial cell clones (ATCC CRL-2160, ATCC CRL-2161, ATCC CRL-2162, ATCC CRL-2163, ATCC CRL-2167, ATCC CRL-2168 and ATCC CRL-2171)
For more information, please contact Cobioer (4008-750-250).
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