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NCI-H446(小细胞肺癌)

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NCI-H446(小细胞肺癌)

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产品编号
CBP61025
货号:
CBP61025
品牌:
Cobioer
规格:
T-25 Flask
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CBP61025
I. General information 
Synonyms: NCI-H446
Background: The NCI-H446 cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with small cell cancer of the lung.
Species: Homo sapiens, human
Tissue: lung; derived from metastatic site: pleural effusion
Disease: carcinoma; small cell lung cancer
Gender: male, 61 years,Caucasian
Morphology: epithelial
Growth Mode: mixed, adherent and suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 13,14
D13S317: 8
D16S539: 12
D5S818: 11
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 18,19
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: Yes, in nude mice
(The cells form transplantable tumors with non-typical SCLC histology.)
Comments: The morphology of the original tumor was not characteristic of SCLC.
The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase.
It does not have detectable levels of L-DOPA decarboxylase, bombesin, vasopressin, oxytocin or gastrin releasing peptide.
C-myc DNA sequences are amplified about 20 fold, and there is a 15 fold increase in c-myc RNA relative to normal cells.
The line was originally propagated in serum free RPMI 1640 medium supplemented with 10 nM hydrocortisone, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 10 nM 17-beta-estradiol, and 30 nM sodium selenite.
The cells form transplantable tumors with non-typical SCLC histology.
For more information, please contact Cobioer (4008-750-250).
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