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1.1B4(杂交细胞系1.1B4通过用人胰腺癌细胞系PANC-1电融合人胰岛的原代培养物而形成。)

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1.1B4(杂交细胞系1.1B4通过用人胰腺癌细胞系PANC-1电融合人胰岛的原代培养物而形成。)

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产品编号
CBP61117
货号:
CBP61117
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
胰腺
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CBP61117
I. General information 
Synonyms: 1.1B4
Background: The hybrid cell line1.1B4 was formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802).
According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay (see protocol below). 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology
The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Species: human (Homo sapiens)
Tissue: Pancreatic islets
Disease: A human pancreatic Beta cell: PANC-1 hybrid cell line. Insulin-secreting cell line.
Gender: N/A
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8
THO1: 7,8
TPOX: 8,11
vWA: 15
Cobioer’s Cell Line Authentication Service
Culture Medium: 1640+10%FBS+5μg/ml Hydrocortisone + 10μg/ml Insulin
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Modal chromosome number 67-71
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm² can be expected.
Comments: For more information, please contact Cobioer (4008-750-250).
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