|I. General information|
|Background:||The 9L/lacZ cell line was developed in 1989 from the 9L cell line (rat nitrosourea induced gliosarcoma cell line).
9L cells were infected with the BAG replication deficient retroviral vector carrying the E. coli lacZ gene encoding beta-gal and the Tn5 neomycin gene, which confers resistance to G418.
The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.
9L/lacZ produced high levels of the enzyme, and was selected for study.
|Species:||Rattus norvegicus, rat|
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|Culture Medium:||DMEM+1 mM sodium pyruvate+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Genes Expressed:||beta galactosidase (beta-gal)|
|Tumor Formation:||Yes, forms tumors in the brains of CD Fischer 344 rats|
|Comments:||The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.
The contrast between stained cells and background facilitates image analysis.
The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture.
For more information, please contact Cobioer (4008-750-250).