|I. General information|
|Background:||The cell line was established by ATCC in 2003 from embryonic day 14 (E14) DR4 mouse embryos obtained from The Jackson Laboratory (Stock# 003208). Mouse embryo fibroblasts (MEFs) prepared from the DR4 mouse strain are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine.
Dr. Rudolf Jaenisch at the Massachusetts Institute of Technology developed the DR4 mouse strain by the intercrossing of three different strains: one bearing resistance genes neoR and puroR, a second bearing the resistance gene hygR, and a third bearing a natural deletion encompassing the Hprt gene. A series of matings incorporated all 4 drug resistance genes into the strain (Tucker et al., 1997; PubMed: 9278500). The background of the original DR4 strain was a mix of 129S4/SvJae, 129P2/OlaHsd, BALB/c, and C57BL/6.
|Species:||Mus musculus, mouse|
|Gender:||Male and female mixed， 14 days gestation embryo|
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|Comments:||The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated and treated the cells with Mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 7 (P7).
ATCC tested that this cell line is resistant to:
G 418 (neomycin): 200 microgm/mL
Puromycin: 0.4 microgm/mL
Hygromycin: 110 microgm/mL
6-Thioguanine: 2.5 microgm/mL
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