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LAMA-84(人急变期慢性粒细胞白血病)

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LAMA-84(人急变期慢性粒细胞白血病)

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产品编号
CBP61153
货号:
CBP61153
品牌:
Cobioer
规格:
T-25 Flask
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其他
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CBP61153
I. General information 
Synonyms: LAMA-84
Background: established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) (treated 5 years with busulfan from 1979-1984) one month after onset of myeloid-megakaryocytic blast crisis; cells were described in the literature to express megakaryocytic and erythroid markers and to respond to induction of differentiation with various reagents; parental cell line of subclone LAMA-87 (DSM ACC 270); cells carry t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene
Species: human (Homo sapiens)
Tissue: N/A 
Disease: chronic myeloid leukemia in blast crisis
Gender: N/A 
Morphology: single, round to polymorph cells growing in suspension, some cells are loosely adherent with a spindle-like morphology;
Growth Mode: N/A 
Doubling Time: ca. 50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD11b +, CD13 +, CD15 -, CD19 -, CD33 +; 
Fingerprint: unique DNA profile using multiplex PCR at D1S80, D2S44, D17S5 and ApoB
Species: confirmed as human with IEF of AST, MDH
Cytogenetics: human hypertriploid karyotype with 3.6% polyploidy - 73/74(69-77)<3n>XX, -X, +1, -2, +5, +6, +8, +13, -14, +17, +17, -18, +22, +mar, del(7)(p15), der(9)t(9;22)(q34;q11)x2, i(11q), add(13)(q33), del(17)(p12), der(22)t(9;22)(q34;q11)x4 - carries Ph (4 copies) - resembles published karyotype
Molec. Genetics: expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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