|I. General information|
|Background:||Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.|
|Species:||Canis familiaris, dog|
Cobioer’s Cell Line Authentication Service
|Culture Medium:||DMEM:Ham's F12(1:1)+0.5 mM sodium pyruvate+0.05 mM non-essential amino acids+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Comments:||Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate).
For tubule formation, the cells should be fed twice a day by a complete medium change.
Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK.
For more information, please contact Cobioer (4008-750-250).