|I. General information|
|Background:||FTC-238 was established from a lung metastasis of a follicular thyroid carcinoma from a 42-year-old male. The cells are polymorphic showing flat polygonal to spindle-like morphologies. Complex chromosomal changes were detected as well as a p53 mutation.
FTC-133 (ECACC Catalogue number 94060901), FTC-236 (ECACC Catalogue number 06030202) and FTC-238 (ECACC Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.
|Species:||human (Homo sapiens)|
|Tissue:||Thyroid (lung metastasis)|
|Disease:||Human follicular thyroid carcinoma, lung metastasis|
|DNA Profile:||Amelogenin: X
Cobioer’s Cell Line Authentication Service
|Culture Medium:||DMEM：Ham's F12(1:1)+ 5％FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Comments:||For more information, please contact Cobioer (4008-750-250).|