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科研细胞

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GLP1R/CRE-Luc/HEK293

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产品编号
CBP71117
货号:
CBP71117
品牌:
Cobioer
规格:
T-25 Flask
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CBP71117
I. Background

GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta
cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis
and release of insulin.  Consequently, GLP1R has been suggested as a potential target for the treatment of
diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice
which over express GLP1R display improved memory and learning.

 
II. Introduction
Host Cell:

HEK293

Expressed gene: Genbank Accession Number NM_002062; no expressed tags
Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):

GLP-1

Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+400ug/ml G418
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for GLP1R receptor

Transducer:  Gs
 
Ⅲ. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅳ.Representative Data

Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular luciferase in HEK293/GLP1R/Luc and HEK293 cells. 

After stimulation with GLP1 receptor agonist, GLP-1 (7-37), the cells are checked with BrightGlo™Luciferase Assay System and the relative luminescence units (RLU) of

 luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10 -fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) co-expressing with Luc 

in HEK293 cells was 4.91 nM. 

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