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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
c-Kit N822K/BaF3
CBP73279 |
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I. Introduction | |
Cell Line Name: |
c-Kit N822K/BaF3 |
Host Cell: |
Ba/F3 |
Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: |
90% FBS+10% DMSO |
Complete Culture Medium: |
RPMI-1640+10%FBS+2ug/ml puromycin |
Mycoplasma Status: |
Negative |
II.Background | |
KIT N822K lies within the activation loop in the protein kinase domain of the Kit protein (PMID: 24205792). N822K results in constitutive activation of Kit, increased Erk1/2 and Stat3 phosphorylation, is transforming in culture (PMID: 24205792, PMID: 31484543), leads to mislocalization of Kit to endolysosomes (PMID: 31484543), and has been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160). |
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III. Representative Data | |
1.WB of c-Kit N822K/BaF3
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2. Sanger of c-Kit N822K/BaF3
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3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT N822K(C4). |
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