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科研细胞

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CRE-Luc/HEK293

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.CBP74027
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.CBP74027
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Cobioer
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T-25 Flask
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.CBP74027
I. Background
The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. It is the main binding site of cAMP response element binding protein (CREB) and is responsible for its activation. CRE is the target of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms. In the cAMP/PKA signaling pathway, CREB is activated via phosphorylation of PKA and binds to CRE with a general motif of 5’-TGACGTCA-3’. Since CRE is a modulator of the cAMP/PKA signaling pathway, it allows the effects of various inhibitors to be studied.
 
II. Description
The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) – HEK293 Cell Line is designed for monitoring the activity of the cAMP/ PKA signaling pathway. The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into HEK293 cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase.
 
III. Introduction
Host Cell: HEK293
Expressed gene: CRE-Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): CRE cell line, CREB cell line, luciferase reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+150ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): • Monitor cAMP/PKA signaling pathway activity.
• Screen for activators or inhibitors of the cAMP/PKA signaling pathway.
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/CRE Luciferase Reporter cells were stimulated by Forsklin, the S/B was 433-fold.

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