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科研细胞

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NFAT-Luc/HEK293

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产品编号
.CBP74028
货号:
.CBP74028
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Cobioer
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T-25 Flask
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CBP74028
I. Background
The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins.
 
II. Description
This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA.
 
III. Introduction
Host Cell: HEK293
Expressed gene: NFAT-Luciferase
Stability: 32 passages
Synonym(s): Nuclear factor of activated T cells; T cell
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10% FBS+300 ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid
Growth Properties: Adherent
 
Ⅴ. Representative Data

Figure 1. NFAT Luciferase Reporter - HEK293 Recombinant Cell Line (C8).

Figure 2. NFAT Luciferase Reporter -HEK293 Recombinant Cell Line (C4).

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