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科研细胞

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SRE-Luc/HEK293

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产品编号
.CBP74030
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.CBP74030
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Cobioer
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T-25 Flask
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.CBP74030
I. Background
The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
 
II. Description
The SRE Reporter – HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter – HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.
 
III. Introduction
Host Cell: HEK293
Expressed gene: SRE-Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): SRE cell line, MAPK reporter, ERK reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+200ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): • Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
• Screen for activators or inhibitors of the MAPK/ERK signaling pathway.
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SRE Luciferase Reporter cells were stimulated by EGF, the S/B was 10-fold.

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