|The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.|
|The SRE Reporter – HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter – HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.|
|Stability:||32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||SRE cell line, MAPK reporter, ERK reporter|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||DMEM+10%FBS+200ug/ml hygromycin|
|Application(s):||• Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
• Screen for activators or inhibitors of the MAPK/ERK signaling pathway.
|IV. Description of Host Cell Line|
|Organism:||Homo sapiens, human|
|Ⅴ. Representative Data|
Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SRE Luciferase Reporter cells were stimulated by EGF, the S/B was 10-fold.