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41BB/NFkB-Luc/HEK293

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产品编号
CBP74045
货号:
CBP74045
品牌:
Cobioer
规格:
T-25 Flask
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CBP74045
I. Background

Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.

 
II. Description

Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry.

 
III. Introduction
Host Cell: HEK293
Expressed gene:

4-1BB

Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): 4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:

MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin

Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid
Growth Properties: Adherent
 
Ⅴ. Representative Data

Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of 4-1BB.

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. 4-1BB/NF-κB Reporter-HEK293 cells were stimulated by 4-1BBL, the S/B was 14.1-fold.

 

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  • Manual download:CBP74045 41BB NFkB-Luc HEK293.pdf

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    2022-07-29
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