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41BB/NFkB-Luc/Jurkat

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产品编号
CBP74062
货号:
CBP74062
品牌:
Cobioer
规格:
T-25 Flask
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CBP74062
I. Background
Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.
 
II. Description

The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.

 
III. Introduction
Host Cell: NFkB reporter Jurkat cells
Expressed gene: 41BB
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): 4-1BB, CD137
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Useful for studying protein binding and screening small molecules.
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 2. 4-1BB/Jurkat (C43) NFkB-Luc report assay stimulated by 4-1BBL.

 

 

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  • Manual download:41BB NFkB-LucJurkat CBP74062.pdf

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    2022-04-21
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