|Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.|
The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.
|Host Cell:||NFkB reporter Jurkat cells|
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Useful for studying protein binding and screening small molecules.|
|IV. Description of Host Cell Line|
|Organism:||Homo sapiens, human|
|Disease:||Acute T cell leukemia|
|Ⅴ. Representative Data|
Figure 2. 4-1BB/Jurkat (C43) NFkB-Luc report assay stimulated by 4-1BBL.