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BCMA/NFkB-Luc/HEK293

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产品编号
CBP74072
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CBP74072
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Cobioer
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T-25 Flask
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CBP74072
I. Background
B-Cell Maturation Antigen (BCMA), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17) or CD269, is a type I membrane protein encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily, that recognizes its ligands including BAFF (B-cell activating factor) and APRIL (a proliferation inducing ligand). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. In vitro, the activation of BCMA by its ligand promotes the differentiation and proliferation of B cells. A vast array of intracellular activity is involved in BCMA-induced signal transduction, including activation of the NF-κB signaling pathway. The bioactivity of BAFF and APRIL as soluble homotrimers distinguishes them from other TNFSF ligands such as TRAIL, FasL, and CD40L, which are only active as membrane-bound molecules.
 
II. Description

The BCMA / NF-κB - HEK293 recombinant cell line has been transfected with full-length human BCMA cDNA (Genbank #NM_001192) under control of a CMV promoter for high constitutive expression. The NF-κB–luciferase reporter is also stably integrated into the genome. The firefly luciferase gene is controlled by four copies of the NF-κB Response Element upstream of a minimal promoter. Upon ligand binding, active BCMA will initiate the NF-κB signaling pathway, leading to expression of the NF-κB-controlled luciferase reporter.

 
III. Introduction
Host Cell: HEK293
Expressed gene: BCMA
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): tumor necrosis factor receptor superfamily member 17, B-Cell Maturation Antigen, TNFRSF17, CD269
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +100ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
 
IV. Description of Host Cell Line
Organism: Human
Tissue: Kidney
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

 

Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of BCMA.

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293-BCMA-NFκB Luciferase Reporter cells were stimulated by rhBAFF, the EC50 was 34.6ng/ml.

 
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  • Manual download:BCMA NFkB-LucHEK293 CBP74072.pdf

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    2022-04-21
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