|CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, antigen presenting cells (APCs) such as monocytes, basophils, dendritic cells and non-immune cells like endothelial cells and epithelial cells have been found to express CD40. A wide variety of carcinoma cells also over-express CD40. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Furthermore, agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC) and promote anti-tumor T-cell responses in addition to fostering cytotoxic myeloid cells with the potential to control cancer in the absence of T-cell immunity.|
Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). This NF-kB luciferase reporter construct is stably integrated into the genome.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+1ug/ml puromycin+100ug/ml hygromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Ⅴ. Representative Data|
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40.
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293-CD40-NFκB Luciferase Reporter cells were stimulated by CD40L, the EC50 was 43ng/ml.