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CD70/CHO

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产品编号
CBP74008
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CBP74008
品牌:
Cobioer
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T-25 Flask
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CBP74008
I. Background
CD70, also known as TNFSF7, is a cytokine which binds to CD27. CD70 is involved in T-cell activation and induces the proliferation of costimulated T-cells. Moreover, TNFSF7 enhances the generation of cytolytic T-cells. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. Diseases associated with CD70 include acute myocarditis, arthritis, and other inflammatory disorders.
 
II. Description
Recombinant CHO cells constitutively expressing human CD70 (also known as Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, and CD27LG; GenBank accession #NM_001252).
 
III. Introduction
Host Cell: CHO
Expressed gene: CD70
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, CD27LG
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+400ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): This cell line is useful for CD70 binding assays, flow cytometry, or for screening for CD70 antibodies. Also useful for immune checkpoint co-stimulatory assay when co-cultured with CD27/NF-κB Reporter-Jurkat cell line (BPS Bioscience #79509).
 
IV. Description of Host Cell Line
Organism: Cricetulus griseus, hamster, Chinese
Tissue: Ovary
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

Figure 1. Recombinant CHO cells constitutively expressing human CD70.

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