Colony Stimulating Factor 1 Receptor (CSF1R, CD115, M-CSF-R) is the receptor for the cytokine CSF1 (MCSF; CSF-1), which controls the growth, function, and differentiation of macrophages. This interaction drives the development and survival of Tumor-Associated Macrophages (TAMs), which in turn suppress the local immune response to the cancer. An alternative ligand is IL-34, which has been implicated in neuroprotective and survival signals following brain injury and neurodegeneration.
As a tyrosine kinase transmembrane receptor, activation of CSF1R by its ligand initiates a vast array of intracellular activity, including activation of the MAPK/ERK signaling pathway. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
DMEM +10%FBS+200ug/ml hygromycin+1ug/ml puromycin
|Application(s):||• Screen for activators or inhibitors of the CSF1R/MAPK/ERK signaling pathway.|
|Ⅲ. Description of Host Cell Line|
|Ⅳ. Representative Data|
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of SRE response elements with constitutive expression of CSF1R(Colony Stimulating Factor 1 Receptor)
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/CFS1R-SRE Luciferase Reporter cells were stimulated by CSF1, the EC50 was 3.75ng/ml.
Figure 3. Inhibition of CSF1 (100 ng/ml) -induced SRE reporter actity by CSF1R inhibitors.