|CTLA4 is a member of the immunoglobulin superfamily. It is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is homologous to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells. CTLA-4 binds CD80 and CD86 with greater affinity and avidity than CD28 thus enabling it to outcompete CD28 for its ligands and act as an “off” switch when bound to CD80 or CD86. Not surprisingly, CTLA-4 is an important drug target for the regulation of the host’s response to cancer.|
Recombinant CHO stably expressing human CTLA4 (Cytotoxic T-Lymphocyte Associated Protein; CD152), GenBank Accession #NM_005214.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||Cytotoxic T-lymphocyte-associated protein 4, CD152|
|Freeze Medium:||90% FBS+10% DMSO|
|Mycoplasma Testing:||The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool, #B3903) to confirm the absence of Mycoplasma species.|
|Application(s):||• Screening for CTLA4 binding molecules (such as anti-CTLA4 antibody) in a cellular context|
|IV. Description of Host Cell Line|
|Organism:||Cricetulus griseus, hamster, Chinese|
|Ⅴ. Representative Data|
Figure 1. Recombinant CHO stably expressing human CTLA4 (Cytotoxic T-Lymphocyte Associated Protein; CD152), GenBank Accession #NM_005214.