CTLA4/IL2 Promoter Reporter/Jurkat
CTLA4 is a member of the immunoglobulin superfamily. It is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is homologous to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells.
|Recombinant Jurkat T cell expressing firefly luciferase gene under the control of IL-2 promoter with constitutive expression of human CTLA4.|
|Expressed gene:||IL2 Promoter|
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
RPMI-1640+10%FBS+1ug/ml puromycin+10ug/ml blasticidin
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Organism:||Homo sapiens, human|
|Disease:||Childhood T acute lymphoblastic leukemia|
|Ⅴ. Representative Data|
Figure 1. Recombinant Jurkat stably expressing human CTLA4.
Figure 2. Dose Response of lpilimumab in CTLA4/IL2 Promoter Reporter-Jurkat Cells (C39) with TCR Activator Cell, the EC50=2.83μg/ml .