|Foxp3, belonging to the forkhead family, is a master transcription factor that expresses exclusively in regulatory T cells, a subset of CD4+ T cells. Regulation of Foxp3 is critical for maintaining immunological tolerance. Over-expression of Foxp3 is known to suppress effector T cell activation.|
Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.
Figure 1. Illustration of Foxp3 promoter region with representative transcription factor binding sites and enhancer regions.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||Foxp3, Foxp3 cell line, scurfin cell line, foxo|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+1 ug/ml puromucin|
|Application(s):||Functional(Report Gene) Assay|
IV. Description of Host Cell Line
|Organism:||Homo sapiens, human|
|Disease:||Acute T cell leukemia|
|Ⅴ. Representative Data|
Figure 2. Foxp3 Jurkat Reporter Activity in response to PMA with lonomycin.