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GITR/NFκB-Luc/Jurkat

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产品编号
CBP74012
货号:
CBP74012
品牌:
Cobioer
规格:
T-25 Flask
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CBP74012
I. Background

Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies.

 
II. Description
This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-?B luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells.
 
III. Introduction
Host Cell: Jurkat
Expressed gene:

GITR-NFκB-Luciferase

Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): NFkB, GITR
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s):

The GITR NF-κB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-kB signaling in response to GITR ligand (GITRL).

 
IV. Description of Host Cell Line
Organism: Human
Tissue: Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human GITR.

 

Figure 2.  Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by GITRL/CHO cells, the EC50 was 2634cells.

 

Figure 3. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by hGITRL, the EC50 was 0.15ug/ml.

 

 

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  • Manual download:GITR NFκB-LucJurkat CBP74012.pdf

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    2022-04-21
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