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HVEM/NFκB-Luc/Jurkat

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产品编号
CBP74013
货号:
CBP74013
品牌:
Cobioer
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T-25 Flask
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CBP74013
I. Background

HVEM is a bidirectional switch regulating T-cell activation in a costimulatory or coinhibitory fashion whose outcome depends on the binding partner. HVEM can act as both receptor and ligand, the binding of endogenous ligand LIGHT or agonist antibodies to HVEM delivers a costimulatory signal; LIGHT/HVEM axis are co-stimulatory immune checkpoint molecules extensively studied for cancer immunotherapy;

 
II. Description
The HVEM cell line is a recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator. Following activation by human HVEM ligand LIGHT, NF-?B transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene.
 
III. Introduction
Host Cell: Jurkat
Expressed gene:

HVEM-NFκB-Luciferase

Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s):

•Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context

•Characterize T cell-mediated immune responses of HVEM and its interactions with LIGHT

•Screen co-stimulatory immune checkpoint molecules

 
IV. Description of Host Cell Line
Organism: Human
Tissue: Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human HVEM.

 

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-HVEM-NFκB-Luciferase Reporter cells were stimulated by LIGHT/CHO cells, the S/B was 8.1-fold.

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  • Manual download:HVEM NFκB-LucJurkat CBP74013.pdf

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    2022-04-21
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