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IL2 promoter-Luc/Jurkat

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产品编号
CBP74014
货号:
CBP74014
品牌:
Cobioer
规格:
T-25 Flask
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CBP74014
I. Background
The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.
 
II. Description
The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.
 
III. Introduction
Host Cell: Jurkat
Expressed gene: IL-2-Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+1ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): • Screen for activators or inhibitors of IL-2 signaling pathway in transduced target cells
• Generation of IL-2 promoter luciferase reporter stable cell line
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

 Figure 1. Response of IL -2-Luciferase Reporter Jurkat Cell Line (C29) to Raji TCR Activator Cell Line.

Figure 2. Dose Response of Recombinant hCTLA4-Fc in IL2 Promoter Reporter-Jurkat Cells with TCR Activitor Raji Cells.

Figure 3. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-IL-2 Luciferase Reporter cells were stimulated by anti-CD3, the EC50 was 8.31ng/ml.

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  • Manual download:IL2_promoter-LucJurkat CBP74014.pdf

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    2022-04-21
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