科研细胞

科研细胞



药靶细胞

药靶细胞



标准品

标准品



NGS靶向捕获探针

NGS靶向捕获探针



浏览量:

IL2 promoter-Luc/Jurkat

所属分类

返回列表



零售价

0.0

元

市场价

0.0

元

浏览量:

产品编号

CBP74014

货号:

CBP74014

品牌:

Cobioer

规格:

T-25 Flask

物种:

组织:

数量

库存:

产品描述

参数

产品数据库

CBP74014
I. Background
The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

II. Description
The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.

III. Introduction
Host Cell:	Jurkat
Expressed gene:	IL-2-Luciferase
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+1ug/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Peripheral blood
Disease:	Acute T cell leukemia
Morphology:	Lymphoblast
Growth Properties:	Suspension

Ⅴ. Representative Data
Figure 1. Response of IL -2-Luciferase Reporter Jurkat Cell Line (C29) to Raji TCR Activator Cell Line.
Figure 2. Dose Response of Recombinant hCTLA4-Fc in IL2 Promoter Reporter-Jurkat Cells with TCR Activitor Raji Cells.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Jurkat-IL-2 Luciferase Reporter cells were stimulated by anti-CD3, the EC50 was 8.31ng/ml.