IL4 Effector Reporter Cell
|Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) that are the biologically most active helper cells for B-cells and that also secrete IL-5 and IL-6. The biological activities of IL-4 are mediated by a specific receptor. The extracellular domain of the IL-4 receptor is related to the receptors for EPO, IL-6, and the beta chain of the IL2 receptor (CD124). Two types of IL-4 receptor (IL-4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL-4R-alpha. The type 2 receptor is a heterodimer consisting of IL-4Ralpha and IL-13Ralpha1. IL-4 enhances expression of MHC class 2 antigens on B-cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T-cells. Pretreatment of macrophages with IL-4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma. IL-4 synergises with EPO and G-CSF in the generation of colonies containing granulocytes or erythroid progenitor cells in a colony formation assay.|
|Stability:||32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||DMEM + 10%FBS+100ug/ml hygromycin|
|III. Representative Data|
Figure 1. Dose Response of Recombinant Human IL4 in Human IL4 Effector Reporter Cells(C7).
Figure 2. Inhibition of lL-4-induced Reporter Activity by IL-4R Neutralizing Antibody in IL4 Effector Reporter Cells (Clone7).