南京科佰生物科技有限公司官方网站欢迎您的光临！
免费咨询热线： 4008-750-250
科研细胞
药靶细胞
标准品
NGS靶向捕获探针
ISRE-Luc/NFkB-SEAP/THP-1
| CBP74097 | |
| I. Background | |
|
THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors. |
|
| II. Description | |
|
The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. |
|
| III. Introduction | |
| Host Cell: | THP-1 |
| Expressed gene: | ISRE |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μg/ml of Zeocin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute monocytic leukemia |
| Morphology: | Monocyte |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
|
|
|
扫一扫，关注我们公众号
Copyright ©2021 www.cobioer.com All Rights Reserved. 南京科佰生物科技有限公司版权所有 苏ICP备13038923号