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LAG3/NFAT-Luc/Jurkat

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产品编号
CBP74060
货号:
CBP74060
品牌:
Cobioer
规格:
T-25 Flask
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CBP74060
I. Background

Lymphocyte-activation gene 3 (LAG3, CD223) is a cell surface protein that belongs to immunoglobulin (Ig) superfamily. LAG3 is expressed on activated T cells, natural killer cells, B cells, and plasmacytoid dendritic cells.

 
II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human LAG3 (lymphocyte-activation gene 3, CD223, GenBank Accession # NM_002286).
 
III. Introduction
Host Cell: Jurkat
Expressed gene: NFAT-LAG3-Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): LAG3, LAG-3, CD223, Cell Line, Jurkat
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+1ug/ml puromycin+400ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Human
Tissue: Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human LAG3.

 

Figure 2.  Dose Response of Anti-LAG3 Neutralizing Antibody in LAG3/NFAT Reporter-Jurkat cells.

 

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  • Manual download:CBP74060 LAG3 NFAT-Luc Jurkat.pdf

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