|This PD-1 Cell Line is used to measure the binding of PD-1 to PD-L1/PD-L2. Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, binds to its ligands, PD-L1 and PD-L2, to negatively regulate immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.|
|Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).|
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, PD1/NFAT, PD-1 NFAT, PD-1/NFAT|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Organism:||Homo sapiens, human|
|Disease:||Acute T cell leukemia|
|Ⅴ. Representative Data|
Figure 1.Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).
Figure 2. Dose response curve of anti-PD-L1 antibody on PD-1/PD-L1 Blockade Bioassay Model. The bioassay consists of two genetically engineered cell lines, Jurkat_PD-1_NFAT-Luc Cells and CHO/TCR activitor/PD-L1 Cells. When co-cultured, the PD-1/PD-L1 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L1 interaction is disrupted, TCR activation induces luminescence (via activation of the NFAT pathway) that can be detected and quantified using Bio-Glo™ Reagent.