PVRIG(CD112R) Effector Reporter Cell
PVRIG (CD112R) is an regulatory protein expressed on T-cells which inhibits immune activation when binding to its counterpart on APCs, PVRL2 (CD112, Nectin-2). Since both PVRL2 and PVR can activate DNAM-1 (CD226), PVRIG works synergistically with and separately from TIGIT. PVRL2 is overexpressed in a variety of tumor types, including breast and ovarian cancers, suggesting that its interaction with PVRIG is a major mechanism of immune suppression in those diseases.
Human secreted CD112R, also known as Poliovirus Receptor-related Immunoglobulin Domain
containing Protein (PVRIG), GenBank Accession No. NM_024070, a.a. 41-172, with C-terminal Fc-Fusion-Avi
tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using AviTag technology. MW = 42 kDa.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG, 79270-1, 79270-2|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Representative Data|
Figure 1. Dose Response of PVRIG Blocking Antibody in PVRIG(CD112R) Effector Reporter Cells (Clone 7) With CD112/TCR Activator - CHO Cells.