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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
STAT5-Luc/BaF3
| .CBPB0001 | |
| I. Background | |
| After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene. | |
| II. Description | |
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The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. |
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| III. Introduction | |
| Expressed gene: | STAT5 |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+10ng/ml IL-3+200ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| IV. Representative Data | |
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Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). STAT5 Luciferase Reporter - BaF3 Cells (C18)，the EC50 was 0.23ng/ml. |
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