|After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.|
The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter.
|Stability:||32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+10ng/ml IL-3+200ug/ml hygromycin|
|IV. Representative Data|
Figure 1. STAT5 Luciferase Reporter - BaF3 Cells (C18)，the EC50 was 0.23ng/ml.