The TLR5/NF-kB Leeporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 5 (TLR5) and Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. TLR5 is one of the key innate immune receptors. Functional activity of the cell line has been validated by TLR5 ligand assay, in which upon activation by Flagellin, TLR5 quickly initiates downstream signaling pathway and mediates nuclear translocation of NF-kB.
The TLR5 reporter line can be used for TLR5-dependent functional assays as well as screening of TLR5 agonists or antagonists. The TLR5 reporter cell line has been validated by flow cytometry and ligand dose response assay.
|Stability:||32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||TLR5/NF-kB cell line；|
|Freeze Medium:||90% FBS+10% DMSO|
MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
|IV. Description of Host Cell Line|
|Growth Properties:||Adherent Morphology: Epithelial|
|Ⅴ. Representative Data|
Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. TLR5/NF-kB Reporter-HEK293 cells were stimulated by FLA-ST, the S/B was 545.6-fold.