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TLR5/NFkB-Luc/HEK293

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CBP74053

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CBP74053

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Cobioer

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T-25 Flask

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CBP74053
I. Background
The TLR5/NF-kB Leeporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 5 (TLR5) and Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. TLR5 is one of the key innate immune receptors. Functional activity of the cell line has been validated by TLR5 ligand assay, in which upon activation by Flagellin, TLR5 quickly initiates downstream signaling pathway and mediates nuclear translocation of NF-kB.

II. Description
The TLR5 reporter line can be used for TLR5-dependent functional assays as well as screening of TLR5 agonists or antagonists. The TLR5 reporter cell line has been validated by flow cytometry and ligand dose response assay.

III. Introduction
Host Cell:	HEK293
Expressed gene:	TLR5、NFkB-Luc
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Human
Tissue:	Embryonic kidney
Disease:	Normal
Morphology:	Epitheloid cell
Growth Properties:	Adherent Morphology: Epithelial

Ⅴ. Representative Data

Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). TLR5/NF-kB Reporter-HEK293 cells were stimulated by FLA-ST, the S/B was 545.6-fold.