南京科佰生物科技有限公司官方网站欢迎您的光临!

免费咨询热线: 4008-750-250

搜索
确认
取消

科研细胞

科研细胞

药靶细胞

药靶细胞

标准品

标准品

NGS靶向捕获探针

NGS靶向捕获探针
浏览量:
1000

HCT116 MTAP-/-cell

所属分类
返回列表
1
零售价
0.0
市场价
0.0
浏览量:
1000
产品编号
CBP75002
货号:
CBP75002
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
数量
-
+
库存:
0
产品描述
参数
产品数据库
CBP75002
I. Background
MTAP (Methylthioadenosine Phosphorylase) is a Protein Coding gene. Diseases associated with MTAP include Diaphyseal Medullary Stenosis With Malignant Fibrous Histiocytoma and Bone Sarcoma. Among its related pathways are Cytokine Signaling in Immune system and Interleukin-12 family signaling. Gene Ontology (GO) annotations related to this gene include 1,4-alpha-oligoglucan phosphorylase activity and S-methyl-5-thioadenosine phosphorylase activity.
 
II. Description
HCT116 MTAP -/- cells have been homozygously knocked out of MTAP gene.

 

III. Introduction

Host Cell: HCT116
Knockout
gene:
MTAP,Homo sapiens (human)
Stability: 32 passages
Synonym(s): methylthioadenosine phosphorylase,BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: McCoy’s 5A+10%FBS
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Colon
Disease: Colorectal Carcinoma
Morphology: Epithelial-like small cells growing as monolayer
Growth Properties: Adherent
 
V. Representative Data

1. WB of HCT116 MTAP-/-cell

Figure 1. WB of MTAP expression.
Lane 1: HCT116   20ug
Lane 2: HCT116 MTAP -/-   20ug

2. Sanger of HCT116 MTAP-/-cell

Figure 2. Sanger sequencing of MTAP: p.M140Rfs*6.

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of HCT116 MTAP KO cells.

Figure 4. CTG Proliferation Assay of HCT116 Parental cells.

 
未找到相应参数组,请于后台属性模板中添加
上一个
下一个

相关文件

上一页
1

相关产品推荐

暂时没有内容信息显示
请先在网站后台添加数据记录。

联系我们

地址:南京市秦淮区普天路1号/实验室地址:上海市浦东新区广丹路222号 

邮箱: sales@cobioer.com (销售部) tech@cobioer.com (技术部)

 COBIOER

扫一扫,关注我们公众号

Copyright ©2021 www.cobioer.com All Rights Reserved. 南京科佰生物科技有限公司版权所有 苏ICP备13038923号