|I. General information|
|Species:||Homo sapiens, human|
|Disease:||Melanoma; Previously described as ductal carcinoma|
|DNA Profile:||Amelogenin: X
Cobioer’s Cell Line Authentication Service
DMEM + 10% FBS+10 ug/ml insulin+ 10 ug/ml glutathione
|Genes Expressed:||tubulin; actin|
|Cellular Products:||tubulin; actin|
|Tumor Formation:||No, in immunosuppressed mice
Yes, in semisolid medium
|Comments:||This cell line was originally described as a spindle shaped variant of the parental MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast.
However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast [PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052].
Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line [PubMed ID: 12354931 and PubMed ID: 17004106].
Derivatives of HTB-129 with identities in question:
M4A4, ATCC® CRL-2914
M4A4 GFP, ATCC® CRL-2915
M4A4 LM3-2 GFP, ATCC® CRL-2916
M4A4 LM3-4 CL 16 GFP, ATCC® CRL-2917
NM2C5, ATCC® CRL-2918
NM2C5 GFP, ATCC® CRL-2919
For more information, please contact Cobioer (4008-750-250).