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OE33(人食道腺癌肿瘤细胞)

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OE33(人食道腺癌肿瘤细胞)

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产品编号
CBP60496
货号:
CBP60496
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
食道
数量
-
+
库存:
0
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CBP60496
I. General information 
Synonyms: OE33
Background: The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett's metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.
Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer
Species: human (Homo sapiens)
Tissue: oesophagus
Disease: 73 year old female
Gender: Human Caucasian oesophageal carcinoma
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10
D13S317: 14
D16S539: 12
D5S818: 11
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI1640+10%FBS

OE33完全培养基,# CBP60496M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Aneuploid
Subculture Routine: Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).
Comments: For more information, please contact Cobioer (4008-750-250).
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