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K562(人慢性粒细胞白血病细胞)

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K562(人慢性粒细胞白血病细胞)

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产品编号
CBP60529
货号:
CBP60529
品牌:
Cobioer
规格:
T-25 Flask
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CBP60529
I. General information 
Synonyms: K562
Background: The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.
Species: Homo sapiens, human
Tissue: bone marrow
Disease: leukemia
Gender: female.53 yearss
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

K562完全培养基,# CBP60529M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: CD7 (25%)
Tumor Formation: Yes, in nude mice.  Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells 
Comments: The cell population has been characterized as highly undifferentiated and of the granulocytic series. Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line.
K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series.The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde.Karyological studies on various K-562 sublines have been classified into three groups (A,B,C) by Dimery, et al.The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined.The line is EBNA negative.
For more information, please contact Cobioer (4008-750-250).
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