|I. General information|
|Background:||The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons.|
|Species:||Homo sapiens, human|
|Tissue:||kidney; derived from metastatic site: pleural effusion|
|Disease:||renal cell adenocarcinoma|
|DNA Profile:||Amelogenin: X
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|Culture Medium:||MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
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|Oncogene:||p53 +; pRB +|
|Genes Expressed:||p53 +; pRB +|
|Tumor Formation:||Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)|
|Comments:||Growth is inhibited by human interferon [Hogan, T. F., personal communication].
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