|I. General information|
|Background:||This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.|
|Species:||Cricetulus griseus, hamster, Chinese|
|Growth Mode:||loosely adherent|
|Culture Medium:||Alpha-MEM ribonucleosides and deoxyribonucleosides+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Comments:||The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA.
The population contains Pro+ revertants at low frequency (approximately 1 in 100,000 cells), and should be suitable for complementation studies which require the Pro- marker.
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at low frequency (approximately 1 in 100000 cells), and should be suitable for complementation studies which require the Pro- marker.
For more information, please contact Cobioer (4008-750-250).