|I. General information|
|Background:||RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 in the Laboratory of Immunology at the National Institute of Dental Research from Wistar rat basophilic cells that were maintained as tumors.|
|Species:||Rattus norvegicus, rat|
|Culture Medium:||MEM+1%Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 15%FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Receptor Expression:||FcERI (Fc of IgE)|
|Comments:||These cells have high affinity IgE receptors.
They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores.
RBL-2H3 cells have been the model for studies of structure of FcERI.
Although nearly all lots of fetal bovine serum support the growth of these cells, the cells grown in some lots degranulate better after FcERI aggregation.
Another rat basophil line is available (RBL-1, see ATCC CRL-1378) that does not degranulate.
Histamine release capacity may be seriously reduced after too much subculturing. Ref
For more information, please contact Cobioer (4008-750-250).
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