|I. General information|
|Background:||NK-92MI is an interleukin-2 (IL-2) independent Natural Killer Cell line derived from the NK-92 (ATCC CRL-2407) cell line by transfection. NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The parental cells were transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector by particle-mediated gene transfer. The transfection is stable.|
|Species:||Homo sapiens, human|
|Tissue:||peripheral blood, blood|
|Disease:||malignant non-Hodgkin's lymphoma|
|Gender:||male，50 years， Caucasian|
|Growth Mode:||suspension, multicell aggregates|
|DNA Profile:||D5S818: 12. 13
D13S317: 9, 12
D7S820: 10, 11
D16S539: 11, 12
vWA: 16, 18
THO1: 6, 9.3
CSF1PO: 11, 12
Amelogenin: X, Y
Cobioer’s Cell Line Authentication Service
|Culture Medium:||75% MEM α (GIBCO 12561-056 ) + 12.5% FBS+12.5% horse serum + 0.2 mM inositoll+0.02 mM folic acid+0.05 mM 2-mercaptoethanol
We strongly suggest to purchase the complete medium from Cobioer.
NK-92 and this derivative cell line NK-92MI have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.
The parental IL-2 dependent cell line is available as ATCC CRL-2407 (NK-92). NK-92MI was shown to contain, express, and synthesize the hIL-2.
A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
For more information, please contact Cobioer (4008-750-250).