|I. General information|
|Background:||The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes' stage D|
|Species:||human (Homo sapiens)|
|Disease:||Human colorectal adenocarcinoma, moderately well differentiated, Dukes' stage D|
|Gender:||a 56-year old male|
|DNA Profile:||Amelogenin: X,Y
Cobioer’s Cell Line Authentication Service
|Culture Medium:||IMDM + 10% FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
|Comments:||For more information, please contact Cobioer (4008-750-250).|
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