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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
GLP1R/CRE-Luc/HEK293
| CBP71117 | |
| I. Background | |
|
GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta |
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| II. Introduction | |
| Host Cell: |
HEK293 |
| Expressed gene: | Genbank Accession Number NM_002062; no expressed tags |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): |
GLP-1 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+400ug/ml G418 |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GLP1R receptor |
| Transducer: | Gs |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅳ.Representative Data | |
|
Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular luciferase in HEK293/GLP1R/Luc and HEK293 cells. After stimulation with GLP1 receptor agonist, GLP-1 (7-37), the cells are checked with BrightGlo™Luciferase Assay System and the relative luminescence units (RLU) of luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10 -fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) co-expressing with Luc in HEK293 cells was 4.91 nM. |
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