Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction.
|Stability:||20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||DMEM+10%FBS+400ug/ml G418+ 100 μg/ml Hygromycin B|
|Storage:||Liquid nitrogen immediately upon delivery|
Functional assay for mMRGPRB2 receptor
|Ⅲ. Description of Host Cell Line|
|Ⅳ. Representative Data|
Figure 1. mMRGPRB2/Ga15/HEK293, the EC50 was 1.47μg/ml.