|Cell Line Name：||EGFR G719D/BaF3|
|Stability:||16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Application:||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.|
|Freeze Medium:||90% FBS+10% DMSO|
|Complete Culture Medium:||RPMI-1640+10%FBS+2ug/ml puromycin|
EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.
|III. Representative Data|
1. WB of EGFR G719D/BaF3
2. Anti-proliferation assay
Figure 2. Anti-proliferation assay of three reference compounds on the EGFR G719D/BaF3 Stable Cell Line.